ypqE [2018-07-26 13:04:35]

similar to PTS, EIIA component

locus: BSU22230

pI: 5.29

mw: 17.79 kDa

protein length: 168 aa

gene length: 504 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.2|Phosphotransferase system] → [category|SW 1.2.2.2|Sugar specific PTS proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.4|Phosphorylation on a His residue]

Gene

Coordinates: 2,336,933 → 2,337,439

The protein

Protein family

• [protein|14ED1AF5038F43F3B151FCBABE6CFC5A2DA3AA6E|PtsI] permease, glucose permease (Glc) family [Pubmed|10627040]

Expression and Regulation

Operons

• genes: [gene|06165870CB68E9C2F1546943CEA668C7790A9647|ptsA]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [pubmed|30038046], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• MGNA-A477 (ypqE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/477 NBRP B. subtilis, Japan]
• QB6099 (aphA3), available in [SW|Jörg Stülke]'s lab [Pubmed|10627040]
• BKE22230 (''ypqE''::''ermC'') (available at the [http://www.bgsc.org/ BGSC] and in [SW|Jörg Stülke]'s lab) [pubmed|28189581]
• GP1863 (''ypqE''::''ermC'') (available in [SW|Jörg Stülke]'s lab)
• BKE22230 (Δ[gene|06165870CB68E9C2F1546943CEA668C7790A9647|ptsA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE22230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTATTTTCTCCCTTCT, downstream forward: _UP4_TAAGCAGGGCGTATGCCTTG
• BKK22230 (Δ[gene|06165870CB68E9C2F1546943CEA668C7790A9647|ptsA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK22230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGTATTTTCTCCCTTCT, downstream forward: _UP4_TAAGCAGGGCGTATGCCTTG

References

• 10627040